Bluefin tuna’s essential habitat lies in the northern Gulf of Mexico, from southern Texas across to the Florida Keys. Within this habitat, the main occurrence hotspots are in the western Gulf, south of the Louisiana-Texas continental shelf. Adult bluefin migrate here to spawn every year. The most important portion of this migratory pathway is around the tip of Florida, but another critical portion of the pathway exists between the Yucatan Peninsula and the mouth of the Mississippi River. The most severe threats to bluefin tuna in the Gulf are in the northeast, just south of the Florida Panhandle, and on the northwest coast of Cuba—areas that align with critical habitats and migratory corridors. These locations should be managed more carefully to reduce the present threats to bluefin tuna habitats and migrations.